Synthetic Antibodies Detect Distinct Cellular States of Chromosome Passenger Complex Proteins.

ABSTRACT: High performance affinity reagents are essential tools to enable biologists to profile the cellular location and composition of macromolecular complexes undergoing dynamic reorganization. To support further development of such tools, we have assembled a high-throughput phage display pipeline to generate Fab-based affinity reagents that target different dynamic forms of a large macromolecular complex, using the Chromosomal Passenger Complex (CPC), as an example. The CPC is critical for the maintenance of chromosomal and cytoskeleton processes during cell division. The complex contains 4 protein components: Aurora B kinase, survivin, borealin and INCENP. The CPC acts as a node to dynamically organize other partnering subcomplexes to build multiple functional structures during mitotic progres

SUBMITTER: Ura M 

PROVIDER: S-EPMC9862951 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature