Effects of Defective Unloading and Recycling of PCNA Revealed by the Analysis of ELG1 Mutants.

ABSTRACT: Timely and complete replication of the genome is essential for life. The PCNA ring plays an essential role in DNA replication and repair by contributing to the processivity of DNA polymerases and by recruiting proteins that act in DNA replication-associated processes. The ELG1 gene encodes a protein that works, together with the Rfc2-5 subunits (shared by the replication factor C complex), to unload PCNA from chromatin. While ELG1 is not essential for life, deletion of the gene has strong consequences for the stability of the genome, and elg1 mutants exhibit sensitivity to DNA damaging agents, defects in genomic silencing, high mutation rates, and other striking phenotypes. Here, we sought to understand whether all the roles attributed to Elg1 in genome stability maint

SUBMITTER: Itzkovich Z 

PROVIDER: S-EPMC9863317 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature