Enhanced-Precision Measurement of Glutathionyl Hemoglobin by MALDI-ToF MS.

ABSTRACT: Glutathionyl-hemoglobin (HbSSG) is used as a human biomarker to pinpoint systemic oxidative stress caused by various pathological conditions, noxious lifestyles, and exposure to drugs and environmental or workplace toxicants. Measurement by MALDI mass spectrometry is most frequently used, however, the method suffers from excessive uncontrolled variability. This article describes the improvement of a MALDI-ToF mass spectrometry method for HbSSG measurement through enhanced precision, based on strict control of sample preparation steps and spreadsheet-based data analysis. This improved method displays enhanced precision in the analysis of several hundred samples deriving from studies in different classes of healthy and diseased human subjects. Levels span from 0.5% (lower limit of detection)

SUBMITTER: Rubino FM 

PROVIDER: S-EPMC9863390 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature