Next Generation Sequencing for the Analysis of Parvovirus B19 Genomic Diversity.

ABSTRACT: Parvovirus B19 (B19V) is a ssDNA human virus, responsible for an ample range of clinical manifestations. Sequencing of B19V DNA from clinical samples is frequently reported in the literature to assign genotype (genotypes 1-3) and for finer molecular epidemiological tracing. The increasing availability of Next Generation Sequencing (NGS) with its depth of coverage potentially yields information on intrinsic sequence heterogeneity; however, integration of this information in analysis of sequence variation is not routinely obtained. The present work investigated genomic sequence heterogeneity within and between B19V isolates by application of NGS techniques, and by the development of a novel dedicated bioinformatic tool and analysis pipeline, yielding information on two newly defined paramete

SUBMITTER: Bichicchi F 

PROVIDER: S-EPMC9863757 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature