Serum Starvation Accelerates Intracellular Metabolism in Endothelial Cells.

ABSTRACT: Periods of low energy supply are challenging conditions for organisms and cells during fasting or famine. Although changes in nutrient levels in the blood are first sensed by endothelial cells, studies on their metabolic adaptations to diminished energy supply are lacking. We analyzed the dynamic metabolic activity of human umbilical vein endothelial cells (HUVECs) in basal conditions and after serum starvation. Metabolites of glycolysis, the tricarboxylic acid (TCA) cycle, and the glycerol pathway showed lower levels after serum starvation, whereas amino acids had increased levels. A metabolic flux analysis with ¹³C-glucose or ¹³C-glutamine labeling for different time points reached a plateau phase of incorporation after 30 h for ¹³C-glucose and after 8 h

SUBMITTER: Lorenz M 

PROVIDER: S-EPMC9863832 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature