CRISPR/Cas9-Mediated Multiplexed Genome Editing in Aspergillus oryzae.

ABSTRACT: Aspergillus oryzae has great potential and competitive advantages to be developed as an excellent expression system, owing to its powerful protein secretion ability, complex post-translational modification, and safety characteristics. However, the low efficiency of genetic modification and gene function analysis is an urgent problem to be solved in A. oryzae and other filamentous fungal systems. Therefore, establishing efficient genetic transformation and multiplexed genome editing tools is significant for developing A. oryzae expression systems, and revealing its intrinsic mechanisms. In this study, the high-efficiency transformation of A. oryzae was achieved by optimizing the preparation conditions of protoplasts, and the random editing efficiency of the CRISP

SUBMITTER: Li Q 

PROVIDER: S-EPMC9864741 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature