Determination of Conformational and Functional Stability of Potential Plague Vaccine Candidate in Formulation.

ABSTRACT: Generally, protein-based vaccines are available in liquid form and are highly susceptible to instability under elevated temperature changes including freezing conditions. There is a need to create a convenient formulation of protein/peptides that can be stored at ambient conditions without loss of activity or production of adverse effects. The efficiency of naturally occurring biocompatible polymer dextran in improving the shelf-life and biological activity of a highly thermally unstable plague vaccine candidate protein called Low Calcium Response V antigen (LcrV), which can be stored at room temperature (30 ± 2 °C), has been evaluated. To determine the preferential interactions with molecular-level insight into solvent-protein interactions, analytical techniques such asspectroscopy, parti

SUBMITTER: Athirathinam K 

PROVIDER: S-EPMC9865242 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature