Glycolytic Plasticity of Metastatic Lung Cancer Captured by Noninvasive ¹⁸F-FDG PET/CT and Serum ¹H-NMR Analysis: An Orthotopic Murine Model Study.

ABSTRACT: We aim to establish a noninvasive diagnostic platform to capture early phenotypic transformation for metastasis using ¹⁸F-FDG PET and ¹H-NMR-based serum metabolomics. Mice with implantation of NCI-H460 cells grew only primary lung tumors in the localized group and had both primary and metastatic lung tumors in the metastatic group. The serum metabolites were analyzed using ¹H-NMR at the time of PET/CT scan. The glycolysis status and cell proliferation were validated by Western blotting and staining. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of SUV_mean and serum metabolites in metastasis. In the metastatic mice, the SUV_mean of metastatic tumors was significantly higher tha

SUBMITTER: Chung YH 

PROVIDER: S-EPMC9866275 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature