The Pseudomonas aeruginosa RpoH (σ³²) Regulon and Its Role in Essential Cellular Functions, Starvation Survival, and Antibiotic Tolerance.

ABSTRACT: The bacterial heat-shock response is regulated by the alternative sigma factor, σ³² (RpoH), which responds to misfolded protein stress and directs the RNA polymerase to the promoters for genes required for protein refolding or degradation. In P. aeruginosa, RpoH is essential for viability under laboratory growth conditions. Here, we used a transcriptomics approach to identify the genes of the RpoH regulon, including RpoH-regulated genes that are essential for P. aeruginosa. We placed the rpoH gene under control of the arabinose-inducible P_BAD promoter, then deleted the chromosomal rpoH allele. This allowed transcriptomic analysis of the RpoH (σ³²) regulon following a short up-shift in the cellular concentration of RpoH by arabino

SUBMITTER: Williamson KS 

PROVIDER: S-EPMC9866376 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature