Effect of Salts on the Conformational Dynamics of the Cytochrome P450 OleP.
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ABSTRACT:
SUBMITTER: De Sciscio ML
PROVIDER: S-EPMC9867029 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
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Effect of Salts on the Conformational Dynamics of the Cytochrome P450 OleP.
De Sciscio Maria Laura ML Nardi Alessandro Nicola AN Parisi Giacomo G Bulfaro Giovanni G Costanzo Antonella A Gugole Elena E Exertier Cécile C Freda Ida I Savino Carmelinda C Vallone Beatrice B Montemiglio Linda Celeste LC D'Abramo Marco M
Molecules (Basel, Switzerland) 20230113 2
Cytochrome P450 OleP catalytic activity is strongly influenced by its structural dynamic conformational behavior. Here, we combine equilibrium-binding experiments with all-atom molecular dynamics simulations to clarify how different environments affect OleP conformational equilibrium between the open and the closed-catalytic competent-forms. Our data clearly show that at high-ionic strength conditions, the closed form is favored, and, very interestingly, different mechanisms, depending on the ch ...[more]
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