Cytological and Molecular Mechanism of Low Pollen Grain Viability in a Germplasm Line of Double Lotus.

ABSTRACT: Self-fertilization rate is an essential index of lotus reproductive system development, and pollen activity is a key factor affecting lotus seed setting rate. Based on cytology and molecular biology, this study addresses the main reasons for the low self-set rate of double lotus. It takes two different double lotus breeds into consideration, namely 'Sijingganshan' with a low self-crossing rate and 'Jinfurong' with a high self-crossing rate. Cytological analysis results showed that the pollen abortion caused by excessive degradation of tapetum during the single phase was the root cause for the low self-mating rate of double lotus. Subsequent transcriptome analysis revealed that the gene NnPTC1 related to programmed tapetum cell death was significantly differentially expressed during

SUBMITTER: Liu G 

PROVIDER: S-EPMC9867118 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature