Identification of platelet subpopulations in cryopreserved platelet components using multi-colour imaging flow cytometry.

ABSTRACT: Cryopreservation of platelets, at  - 80 °C with 5-6% DMSO, results in externalisation of phosphatidylserine and the formation of extracellular vesicles (EVs), which may mediate their procoagulant function. The phenotypic features of procoagulant platelets overlap with other platelet subpopulations. The aim of this study was to define the phenotype of in vitro generated platelet subpopulations, and subsequently identify the subpopulations present in cryopreserved components. Fresh platelet components (n = 6 in each group) were either unstimulated as a source of resting platelets; or stimulated with thrombin and collagen to generate a mixture of aggregatory and procoagulant platelets; calcium ionophore (A23187) to generate procoagulant platelets; or ABT-737 to generate apoptotic platelets. P

SUBMITTER: Johnson L 

PROVIDER: S-EPMC9867743 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature