Comparing the transcriptome of developing native and iPSC-derived mouse retinae by single cell RNA sequencing.

ABSTRACT: We report the generation and analysis of single-cell RNA-Seq data (> 38,000 cells) from mouse native retinae and induced pluripotent stem cell (iPSC)-derived retinal organoids at four matched stages of development spanning the emergence of the major retinal cell types. We combine information from temporal sampling, visualization of 3D UMAP manifolds, pseudo-time and RNA velocity analyses, to show that iPSC-derived 3D retinal organoids broadly recapitulate the native developmental trajectories. However, we observe relaxation of spatial and temporal transcriptome control, premature emergence and dominance of photoreceptor precursor cells, and susceptibility of dynamically regulated pathways and transcription factors to culture conditions in retinal organoids. We demonstrate that genes causin

SUBMITTER: Georges A 

PROVIDER: S-EPMC9867755 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature