Factorial Design to Optimize Matrix Spraying Parameters for MALDI Mass Spectrometry Imaging.

ABSTRACT: Matrix deposition is a critical step in obtaining reproducible and spatially representative matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging data. To date, few limited studies have examined the optimization of matrix spraying parameters for maximizing analyte extraction while minimizing analyte delocalization. Herein, we present a study using automated pneumatic spraying with a heated sample-holder tray to determine an optimized model for mouse whole kidney lipid imaging using a 2,5-dihydroxybenzoic acid matrix in which the solvent flow rate, nozzle velocity, and sample heating were optimized using a two-level factorial experimental design. Parameters examined to determine the optimum model include the number of analytes, the matrix crystal size, off tissue del

SUBMITTER: Tressler C 

PROVIDER: S-EPMC9867919 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature