Stemness-related genes revealed by single-cell profiling of naive and stimulated human CD34⁺ cells from CB and mPB.

ABSTRACT:

Background

Hematopoietic stem cells (HSCs) from different sources show varied repopulating capacity, and HSCs lose their stemness after long-time ex vivo culture. A deep understanding of these phenomena may provide helpful insights for HSCs.

Methods

Here, we applied single-cell RNA-seq (scRNA-seq) to analyse the naïve and stimulated human CD34⁺ cells from cord blood (CB) and mobilised peripheral blood (mPB).

Results

We collected over 16 000 high-quality single-cell data to construct a comprehensive inference map and characterised the HSCs under a quiescent state on the hierarchy top. Then, we compared HSCs in CB with those in mPB and HSCs of naïve samples to those of cultured samples, and identified stemness-related genes (SRGs) associated with cell source

SUBMITTER: Dong G 

PROVIDER: S-EPMC9868212 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature