Epidermal growth factor regulates autophagy activity and endocytosis of yak cumulus cells in a concentration-dependent manner.

ABSTRACT:

Introduction

Autophagy and endocytosis are crucial biological activities in mammalian follicle development and oocyte maturation, which are easily affected by external environmental factors. Epidermal growth factor (EGF), as an important component of follicular fluid, regulates the growth and apoptosis of follicular cells. However, its regulatory mechanism of autophagy and endocytosis in mammals, especially in large domestic animals such as plateau yak, remains unclear. This study aimed to investigate the regulatory mechanism of EGF on autophagy and endocytosis in yak cumulus cells.

Methods

Yak cumulus cells were treated with different concentrations of EGF and appropriate concentrations of EGFR inhibitor gefitinib (10 μM). The dynamic expression levels of Atg5, Beclin1, LC3

SUBMITTER: Ma R 

PROVIDER: S-EPMC9868291 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature