Transcriptomic profiling analysis of human endometrial stromal cells treated with autologous platelet-rich plasma.

ABSTRACT:

Purpose

To clarify the mechanisms of intrauterine platelet-rich plasma (PRP) infusion that support embryo implantation in in vitro fertilization treatment.

Methods

Blood and endometrial samples were collected from four infertile women. Human endometrial stromal cells (HESCs) were cultured and passaged equally into four cell culture dishes in each patient. Two were treated with PRP twice, and the other two were treated with vehicle. Subsequently, two cultures with and without PRP were decidualized with 8-bromoadenosine 3',5'-cyclic AMP and progesterone for 5 days.

Results

The gene expression in undifferentiated or decidualized HESCs with and without PRP was compared. In the microarray analysis, 381 and 63 differentially expressed genes were detected in undifferentiated

SUBMITTER: Kuroda K 

PROVIDER: S-EPMC9868347 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature