A simplified protocol for DNA extraction from FTA cards for faecal microbiome studies.

ABSTRACT: As metagenomic studies continue to increase in size and complexity, they are often required to incorporate data from geographically isolated locations or longitudinal time samples. This represents a technical challenge, given that many of the commonly used methods used for sample collection, storage, and DNA extraction are sensitive to differences related to the time, storage and chemistry involved. FTA cards have been previously proposed as a simple, reliable and cost-efficient method for the preservation of animal faecal microbiomes. In this study, we report a simplified extraction methodology for recovering microbiome DNA from faeces stored on FTA cards and compare its performance to a common alternative means of characterising such microbiomes; namely, immediate freezing of the faeces

SUBMITTER: Bolt Botnen A 

PROVIDER: S-EPMC9868478 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature