Ferroportin depletes iron needed for cell cycle progression in head and neck squamous cell carcinoma.

ABSTRACT:

Introduction

Ferroportin (FPN), the only identified eukaryotic iron efflux channel, plays an important role in iron homeostasis and is downregulated in many cancers. To determine if iron related pathways are important for Head and Neck Squamous Cell Carcinoma (HNSCC) progression and proliferation, we utilize a model of FPN over-expression to simulate iron depletion and probe associated molecular pathways.

Methods

The state of iron related proteins and ferroptosis sensitivity was assessed in a panel of metastatic HNSCC cell lines. Stable, inducible expression of FPN was confirmed in the metastatic HNSCC lines HN12 and JHU-022 as well as the non-transformed normal oral keratinocyte (NOK) cell line and the effect of FPN mediated iron depletion was assessed in these cell lines.<

SUBMITTER: Belvin BR 

PROVIDER: S-EPMC9868905 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature