CCL3 secreted by hepatocytes promotes the metastasis of intrahepatic cholangiocarcinoma by VIRMA-mediated N6-methyladenosine (m⁶A) modification.

ABSTRACT:

Background

Intrahepatic cholangiocarcinoma (ICC) is a malignant disease characterized by onset occult, rapid progression, high relapse rate, and high mortality. However, data on how the tumor microenvironment (TME) regulates ICC metastasis at the transcriptomic level remains unclear. This study aimed to explore the mechanisms and interactions between hepatocytes and ICC cells.

Methods

We analyzed the interplay between ICC and liver microenvironment through cytokine antibody array analysis. Then we investigated the role of N6-methyladenosine (m⁶A) modification and the downstream target in vitro, in vivo experiments, and in clinical specimens.

Results

Our study demonstrated that cytokine CCL3, which is secreted by hepatocytes, promotes tumor metastasis by reg

SUBMITTER: Zhou S 

PROVIDER: S-EPMC9869516 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature