Role of acyl-coenzyme A: cholesterol transferase 1 (ACAT1) in retinal neovascularization.

ABSTRACT:

Background

We have investigated the efficacy of a new strategy to limit pathological retinal neovascularization (RNV) during ischemic retinopathy by targeting the cholesterol metabolizing enzyme acyl-coenzyme A: cholesterol transferase 1 (ACAT1). Dyslipidemia and cholesterol accumulation have been strongly implicated in promoting subretinal NV. However, little is known about the role of cholesterol metabolism in RNV. Here, we tested the effects of inhibiting ACAT1 on pathological RNV in the mouse model of oxygen-induced retinopathy (OIR).

Methods

In vivo studies used knockout mice that lack the receptor for LDL cholesterol (LDLR^-/-) and wild-type mice. The wild-type mice were treated with a specific inhibitor of ACAT1, K604 (10 mg/kg, i.p) or vehicle (PBS) during

SUBMITTER: Zaidi SAH 

PROVIDER: S-EPMC9869542 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature