Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo.

ABSTRACT: As ribosomes translate the genetic code, they can encounter a variety of obstacles that hinder their progress. If ribosomes stall for prolonged times, cells suffer due to the loss of translating ribosomes and the accumulation of aberrant protein products. Thus to protect cells, stalled ribosomes experience a series of reactions to relieve the stall and degrade the offending mRNA, a process known as No-Go mRNA Decay (NGD). While much of the machinery for NGD is known, the precise ordering of events and factors along this pathway has not been tested. Here, we deploy C. elegans to unravel the coordinated events comprising NGD. Utilizing a novel reporter and forward and reverse genetics, we identify the machinery required for NGD. Our subsequent molecular analyses define a functional requireme

SUBMITTER: Monem PC 

PROVIDER: S-EPMC9870110 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature