Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments.

ABSTRACT: Faithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and complex prototyping, preventing their broad adoption by the scientific community. To make 3D cell culture more available and adaptable we here describe the use of the fused deposition modeling (FDM) technology to rapid-prototype 3D printed perfusion bioreactors. Our 3D printed bioreactors are made of polylactic acid resulting in reusable systems customizable in size and shape. Following design confirmation, our bioreactors were biologically validated for the culture of human mesenchymal stromal cells under perfusion for up to 2 weeks on collagen scaffolds. Microenvironments of variou

SUBMITTER: Dupard SJ 

PROVIDER: S-EPMC9870251 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature