Development of the DNA-based voltammetric biosensor for detection of vincristine as anticancer drug.

ABSTRACT: In the article presented herein, a deoxyribonucleic acid (DNA) biosensor is introduced for Vincristine determination in pharmaceutical preparations based on the modification of screen printed electrode (SPE) with double-stranded DNA (ds-DNA), polypyrrole (PP), peony-like CuO:Tb³⁺ nanostructure (P-L CuO:Tb³⁺ NS). The developed sensor indicated a wide linear response to Vincristine concentration ranged from 1.0 nM to 400.0 μM with a limit of detection as low as .21 nM. The intercalation of Vincristine with DNA guanine led to the response. The optimized parameters for the biosensor performance were ds-DNA/Vincristine interaction time, DNA concentration and type of buffer solution. The docking investigation confirm the minor groove interaction between guanine base at surf

SUBMITTER: Abbasi M 

PROVIDER: S-EPMC9870317 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature