Salivary Areca and tobacco alkaloids for bioverification in the Betel Nut Intervention Trial.

ABSTRACT:

Background

The Betel Nut Intervention Trial (BENIT) is the first known randomized controlled intervention trial designed to help minority Pacific Islanders in Guam and Saipan quit chewing the carcinogenic Areca catechu nut (AN). We report the BENIT's saliva bioverification results against the self-reported chewing status ("quitter" or "chewer") at day 22 follow-up.

Material and methods

AN-specific (arecoline, arecaidine, guvacoline, and guvacine) and tobacco-specific (nicotine, cotinine, and hydroxycotinine) alkaloids were analyzed in saliva from 176 BENIT participants by an established and sensitive liquid chromatography mass spectrometry-based assay.

Results

The combined four AN alkaloid levels decreased from baseline in quitters (n = 50) and chewers (n = 108) by 32

SUBMITTER: Franke AA 

PROVIDER: S-EPMC9870849 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature