Complementary measurement of nontyphoidal Salmonella-specific IgG and IgA antibodies in oral fluid and serum.

ABSTRACT:

Objectives

Immuno-epidemiological studies of orally acquired, enteric pathogens such as nontyphoidal Salmonella (NTS) often focus on serological measures of immunity, ignoring potentially relevant oral mucosal responses. In this study we sought to assess the levels and detectability of both oral fluid and serum IgG and IgA to NTS antigens, in endemic and non-endemic populations.

Methods

IgG and IgA antibodies specific for Salmonella Typhimurium and Salmonella Enteritidis O antigen and phase 1 flagellin were assessed using Enzyme Linked Immunosorbent Assay (ELISA). Paired oral fluid and serum samples were collected from groups of 50 UK adults, Kenyan adults and Kenyan infants. Additionally, oral fluid alone was collected from 304 Kenyan individuals across

SUBMITTER: Elias SC 

PROVIDER: S-EPMC9871079 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature