Aptamer-coated track-etched membranes with a nanostructured silver layer for single virus detection in biological fluids.

ABSTRACT: Aptasensors based on surface-enhanced Raman spectroscopy (SERS) are of high interest due to the superior specificity and low limit of detection. It is possible to produce stable and cheap SERS-active substrates and portable equipment meeting the requirements of point-of-care devices. Here we combine the membrane filtration and SERS-active substrate in the one pot. This approach allows efficient adsorption of the viruses from the solution onto aptamer-covered silver nanoparticles. Specific determination of the viruses was provided by the aptamer to influenza A virus labeled with the Raman-active label. The SERS-signal from the label was decreased with a descending concentration of the target virus. Even several virus particles in the sample provided an increase in SERS-spectra intensity, re

SUBMITTER: Kukushkin V 

PROVIDER: S-EPMC9871243 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature