Identification of selective hepatitis delta virus ribozyme inhibitors by high-throughput screening of small molecule libraries.

ABSTRACT:

Background & aims

Chronic hepatitis delta is the most severe form of chronic viral hepatitis and is associated with faster progression towards cirrhosis, liver decompensation, and hepatocellular carcinoma. Hepatitis delta virus (HDV)'s tight dependency on hepatitis B virus and the host cell machinery for its life cycle limits the development of direct-acting antivirals. Thus, we aimed to identify compounds that could block HDV replication by targeting its antigenomic ribozyme.

Methods

We generated stable Huh7 human hepatoma cells expressing a reporter gene (Gaussia luciferase) either downstream (Gluc-2xRz) or upstream (2xRz-Gluc) of two HDV antigenomic ribozyme sequences. We performed high-throughput screening of three small molecule libraries. The secreted luciferase was me

SUBMITTER: Tseligka ED 

PROVIDER: S-EPMC9871325 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature