Validation of carbon isotopologue distribution measurements by GC-MS and application to ¹³C-metabolic flux analysis of the tricarboxylic acid cycle in Brassica napus leaves.

ABSTRACT: The estimation of metabolic fluxes in photosynthetic organisms represents an important challenge that has gained interest over the last decade with the development of ¹³C-Metabolic Flux Analysis at isotopically non-stationary steady-state. This approach requires a high level of accuracy for the measurement of Carbon Isotopologue Distribution in plant metabolites. But this accuracy has still not been evaluated at the isotopologue level for GC-MS, leading to uncertainties for the metabolic fluxes calculated based on these fragments. Here, we developed a workflow to validate the measurements of CIDs from plant metabolites with GC-MS by producing tailor-made E. coli standard extracts harboring a predictable binomial CID for some organic and amino acids. Overall, most of our T

SUBMITTER: Dellero Y 

PROVIDER: S-EPMC9871494 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature