Arbitrary Ca²⁺ regulation for endothelial nitric oxide, NFAT and NF-κB activities by an optogenetic approach.

ABSTRACT: Modern western dietary habits and low physical activity cause metabolic abnormalities and abnormally elevated levels of metabolites such as low-density lipoprotein, which can lead to immune cell activation, and inflammatory reactions, and atherosclerosis. Appropriate stimulation of vascular endothelial cells can confer protective responses against inflammatory reactions and atherosclerotic conditions. This study aims to determine whether a designed optogenetic approach is capable of affecting functional changes in vascular endothelial cells and to evaluate its potential for therapeutic regulation of vascular inflammatory responses in vitro. We employed a genetically engineered, blue light-activated Ca²⁺ channel switch molecule that utilizes an endogenous store-operated ca

SUBMITTER: Yamanaka T 

PROVIDER: S-EPMC9871596 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature