Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians.

ABSTRACT: Peripheral blood immune cells have different molecular characteristics at different stages of the whole lifespan. Knowledge of circulating immune cell types and states from children to centenarians remains incomplete. We profiled peripheral blood mononuclear cells (PBMCs) of multiple age groups with single-cell RNA sequencing (scRNA-seq), involving the age ranges of 1-12 (G1), 20-30(G2), 30-60(G3), 60-80(G4), and >110 years (G5). The proportion and states of myeloid cells change significantly from G1 to G2. We identified a novel CD8⁺CCR7⁺GZMB⁺ cytotoxic T cell subtype specific in G1, expressing naive and cytotoxic genes, and validated by flow cytometry. CD8⁺ T cells showed significant changes in the early stage (G1 to G2), while CD4⁺ T

SUBMITTER: Zhong J 

PROVIDER: S-EPMC9871912 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature