Enhanced detection sensitivity of neuronal activity patterns using CaMPARI1 vs. CaMPARI2.

ABSTRACT: Calcium-modulated photoactivatable ratiometric integrator (CaMPARI) is a calcium ion (Ca²⁺)- and light-dependent genetically encoded fluorescent activity integrator that can capture snapshots of neuronal activity through an irreversible process known as photoconversion. This unique property was previously used to label neurons based upon their tuning properties in order to map synaptic connectivity and to record large-scale neuronal activity in freely moving mice without attaching any mechanical device to them. The latest version of CaMPARI (CaMPARI2) was engineered to enhance the contrast generated by photoconverting the green protein to the activity-dependent red form and to reduce the Ca²⁺-independent photoconversion rate compared to the first generation of CaMPARI

SUBMITTER: Das A 

PROVIDER: S-EPMC9871923 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature