Widespread, Reversible Cysteine Modification by Methylglyoxal Regulates Metabolic Enzyme Function.

ABSTRACT: Methylglyoxal (MGO), a reactive metabolite byproduct of glucose metabolism, is known to form a variety of posttranslational modifications (PTMs) on nucleophilic amino acids. For example, cysteine, the most nucleophilic proteinogenic amino acid, forms reversible hemithioacetal and stable mercaptomethylimidazole adducts with MGO. The high reactivity of cysteine toward MGO and the rate of formation of such modifications provide the opportunity for mechanisms by which proteins and pathways might rapidly sense and respond to alterations in levels of MGO. This indirect measure of alterations in glycolytic flux would thereby allow disparate cellular processes to dynamically respond to changes in nutrient availability and utilization. Here we report the use of quantitative LC-MS/MS-based chemoprot

SUBMITTER: Coukos JS 

PROVIDER: S-EPMC9872086 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature