Macrophages direct cancer cells through a LOXL2-mediated metastatic cascade in pancreatic ductal adenocarcinoma.

ABSTRACT:

Objective

The lysyl oxidase-like protein 2 (LOXL2) contributes to tumour progression and metastasis in different tumour entities, but its role in pancreatic ductal adenocarcinoma (PDAC) has not been evaluated in immunocompetent in vivo PDAC models.

Design

Towards this end, we used PDAC patient data sets, patient-derived xenograft in vivo and in vitro models, and four conditional genetically-engineered mouse models (GEMMS) to dissect the role of LOXL2 in PDAC. For GEMM-based studies, K-Ras ^+/LSL-G12D;Trp53 ^LSL-R172H;Pdx1-Cre mice (KPC) and the K-Ras ^+/LSL-G12D;Pdx1-Cre mice (KC) were crossed with Loxl2 allele floxed mice (Loxl2^{Exon2 fl/fl}) or conditional Loxl2 overex

SUBMITTER: Alonso-Nocelo M 

PROVIDER: S-EPMC9872246 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature