Activation of NLRP3 inflammasome in lung epithelial cells triggers radiation-induced lung injury.

ABSTRACT:

Background

Radiation-induced lung injury (RILI) is the most common and serious complication of chest radiotherapy. However, reported radioprotective agents usually lead to radiation resistance in tumor cells. The key to solving this problem is to distinguish between the response of tumor cells and normal lung epithelial cells to radiation damage.

Methods

RNA-Seq was used to recognize potential target of alleviating the progression of RILI as well as inhibiting tumor growth. The activation of NLRP3 inflammasome in lung epithelial cells was screened by qRT-PCR, western blotting, immunofluorescence, and ELISA. An in vivo model of RILI and in vitro conditioned culture model were constructed to evaluate the effect of NLRP3/interleukin-1β on fibroblasts activation. ROS, ATP, and (

SUBMITTER: Rao X 

PROVIDER: S-EPMC9872296 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature