Connecting Conformational Motions to Rapid Dynamics in Human Purine Nucleoside Phosphorylase.

ABSTRACT: The influence of protein motions on enzyme catalysis remains a topic of active discussion. Protein motions occur across a variety of time scales, from vibrational fluctuations in femtoseconds, to collective motions in milliseconds. There have been numerous studies that show conformational motions may assist in catalysis, protein folding, and substrate specificity. It is also known through transition path sampling studies that rapid promoting vibrations contribute to enzyme catalysis. Human purine nucleoside phosphorylase (PNP) is one enzyme that contains both an important conformational motion and a rapid promoting vibration. The slower motion in this enzyme is associated with a loop motion, that when open allows substrate entry and product release but closes over the active site during ca

SUBMITTER: Frost CF 

PROVIDER: S-EPMC9873402 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature