Biochemical alterations in the follicular fluid of bovine peri-ovulatory follicles and association with final oocyte maturation.

ABSTRACT: Follicular fluid (FF), a product of vascular transudate and granulosa and thecal cell secretions, is the milieu that has evolved to support oocyte growth and maturation which plays a central role in oocyte quality determination. Therefore, a suboptimal FF composition may be reflected in compromised oocyte progression through maturation, fertilization or embryo development. To date, the composition of bovine FF remains understudied. To address this, we comprehensively characterized the metabolomic constituency of bovine FF in the period during which the oocyte undergoes meiotic maturation. More specifically, FF from pre (-24 h) and peri (-2 h) -ovulatory follicles was profiled by high-throughput untargeted ultra-high-performance liquid chromatography tandem mass spectroscopy. A total of 634

SUBMITTER: Abdulrahman Alrabiah N 

PROVIDER: S-EPMC9874974 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature