The role of PAX1 methylation in predicting the pathological upgrade of cervical intraepithelial neoplasia before cold knife conization.

ABSTRACT:

Objective

To explore the ability of PAX1 methylation (PAX1^m) to predict the pathological upgrade of cervical intraepithelial neoplasia (CIN) before cold knife conization (CKC).

Methods

A total of 218 women that underwent colposcopy-directed biopsy (CDB) pathology for the confirmation of CIN2 and CIN3 between December 2020 to September 2021 were enrolled in this study. The methylation levels of PAX1 (ΔCp_PAX1) were determined by quantitative methylation-specific polymerase chain reaction (qMSP). Receiver operating characteristic curve was used to identify the optimal cut-off value of ΔCp_PAX1 for predicting the pathological upgrade of disease.

Results

In the CDB-confirmed CIN2 group, 36% of CIN2 was found to have pathologically upgraded t

SUBMITTER: Li M 

PROVIDER: S-EPMC9875021 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature