Sequence Tendency for the Interaction between Low-Complexity Intrinsically Disordered Proteins.

ABSTRACT: Reversible interaction between intrinsically disordered proteins (IDPs) is considered as the driving force for liquid-liquid phase separation (LLPS), while the detailed description of such a transient interaction process still remains a challenge. And the mechanisms underlying the behavior of IDP interaction, for example, the possible relationship with its inherent conformational fluctuations and sequence features, remain elusive. Here, we use atomistic molecular dynamics (MD) simulation to investigate the reversible association of the LAF-1 RGG domain, the IDP with ultra-low LLPS concentration (0.06 mM). We find that the duration of the association between two RGG domains is highly heterogeneous, and the sustained associations essentially dominate the IDP interaction. More interestingly,

SUBMITTER: Zhang M 

PROVIDER: S-EPMC9875249 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature