Facile Functionalization of Carbon Electrodes for Efficient Electroenzymatic Hydrogen Production.

ABSTRACT: Enzymatic electrocatalysis holds promise for new biotechnological approaches to produce chemical commodities such as molecular hydrogen (H₂). However, typical inhibitory limitations include low stability and/or low electrocatalytic currents (low product yields). Here we report a facile single-step electrode preparation procedure using indium-tin oxide nanoparticles on carbon electrodes. The subsequent immobilization of a model [FeFe]-hydrogenase from Clostridium pasteurianum ("CpI") on the functionalized carbon electrode permits comparatively large quantities of H₂ to be produced in a stable manner. Specifically, we observe current densities of >8 mA/cm² at -0.8 V vs the standard hydrogen electrode (SHE) by direct electron transfer (DET) from cyclic

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9875370 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature