Dynamic changes in whole genome DNA methylation, chromatin and gene expression during mouse lens differentiation.

ABSTRACT:

Background

Cellular differentiation is marked by temporally and spatially coordinated gene expression regulated at multiple levels. DNA methylation represents a universal mechanism to control chromatin organization and its accessibility. Cytosine methylation of CpG dinucleotides regulates binding of methylation-sensitive DNA-binding transcription factors within regulatory regions of transcription, including promoters and distal enhancers. Ocular lens differentiation represents an advantageous model system to examine these processes as lens comprises only two cell types, the proliferating lens epithelium and postmitotic lens fiber cells all originating from the epithelium.

Results

Using whole genome bisulfite sequencing (WGBS) and microdissected lenses, we investigated dynami

SUBMITTER: Chang W 

PROVIDER: S-EPMC9875507 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature