Proteomic analysis of pharate pupal molting fluid from the tobacco hornworm, Manduca sexta.

ABSTRACT: The insect cuticle is a key component of their success, being important for protection, communication, locomotion, and support. Conversely, as an exoskeleton, it also limits the size of the insect and must be periodically molted and a new one synthesized, to permit growth. To achieve this, the insect secretes a solution of chitinases, proteases and other proteins, known collectively as molting fluid, during each molting process to break down and recycle components of the old cuticle. Previous research has focused on the degradative enzymes in molting fluid and offered some characterization of their biochemical properties. However, identification of the specific proteins involved remained to be determined. We have used 2D SDS-PAGE and LC/MS-based proteomic analysis to identify proteins in t

SUBMITTER: Dittmer NT 

PROVIDER: S-EPMC9875806 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature