Testis-enriched ferlin, FER1L5, is required for Ca²⁺-activated acrosome reaction and male fertility.

ABSTRACT: Spermatozoa need to undergo an exocytotic event called the acrosome reaction before fusing with eggs. Although calcium ion (Ca²⁺) is essential for the acrosome reaction, its molecular mechanism remains unknown. Ferlin is a single transmembrane protein with multiple Ca²⁺-binding C2 domains, and there are six ferlins, dysferlin (DYSF), otoferlin (OTOF), myoferlin (MYOF), fer-1-like 4 (FER1L4), FER1L5, and FER1L6, in mammals. Dysf, Otof, and Myof knockout mice have been generated, and each knockout mouse line exhibited membrane fusion disorders such as muscular dystrophy in Dysf, deafness in Otof, and abnormal myogenesis in Myof. Here, by generating mutant mice of Fer1l4, Fer1l5, and Fer1l6, we found that onl

SUBMITTER: Morohoshi A 

PROVIDER: S-EPMC9876558 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature