Biliary epithelial cell differentiation of bipotent human liver-derived organoids by 2D and 3D culture.

ABSTRACT: Organoid culture is a technology for creating three-dimensional (3D) tissue-like structures in vitro, and is expected to be used in various fields. It was reported that human adult bile duct cells derived from human biopsy can be expanded as organoids in vitro that exhibit stem cell-like properties including high proliferative ability and differentiation ability toward both hepatocytes and biliary epithelial cells (BECs). Although many studies have achieved the efficient differentiation of bipotent human liver-derived organoids (hLOs) toward mature hepatocytes, the differentiation potency toward mature BECs remains unclear. In this study, we attempted to evaluate the differentiation potency of bipotent hLOs, which were generated from primary (cryopreserved) human hepatocytes

SUBMITTER: Tong Y 

PROVIDER: S-EPMC9876776 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature