Isolation of single cells from human hepatoblastoma tissues for whole-exome sequencing.
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ABSTRACT:
SUBMITTER: He J
PROVIDER: S-EPMC9876968 | biostudies-literature | 2023 Mar
REPOSITORIES: biostudies-literature
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Isolation of single cells from human hepatoblastoma tissues for whole-exome sequencing.
He Jian J Meng Mei M Zhou Xianchao X Gao Rui R Wang Hui H
STAR protocols 20230121 1
By combining single-cell processing with whole-exome sequencing, we have developed single-cell whole-exome sequencing to investigate the mechanisms of hepatoblastoma development and to provide potential targets and therapeutic approaches for clinical treatment. In the following protocol, we outline the steps involved in single-cell sorting, whole-genome amplification, amplification uniformity estimation, and whole-exome library construction. In addition to the cells we use, this protocol is also ...[more]
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