Oral microbiome and risk of incident head and neck cancer: A nested case-control study.

ABSTRACT:

Objectives

This nested case-control study in the NIH-AARP Diet and Health Study was carried out to prospectively investigate the relationship of oral microbiome with head and neck cancer (HNC).

Materials and methods

56 incident HNC cases were identified, and 112 controls were incidence-density matched to cases. DNA extracted from pre-diagnostic oral wash samples was whole-genome shotgun metagenomic sequenced to measure the overall oral microbiome. ITS2 gene qPCR was used to measure the presence of fungi. ITS2 gene sequencing was performed on ITS2 gene qPCR positive samples. We computed taxonomic and functional alpha-diversity and beta-diversity metrics. The presence and relative abundance of groups of red-complex (e.g., Porphyromonas gingivalis) and/or orange-complex (e.g.,

SUBMITTER: Wu Z 

PROVIDER: S-EPMC9877180 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature