Identification of genes involved in chicken follicle selection by ONT sequencing on granulosa cells.

ABSTRACT: In chickens, follicle selection is an important process affecting laying traits, which is characterized by the differentiation of granulosa cells and the synthesis of progesterone by granulosa cells from hierarchical follicles. By using Oxford Nanopore Technologies (ONT) approach, we compared the transcriptomes of granulosa cells between pre-hierarchical (Pre-GCs) and hierarchical follicles (Post-GCs) to identify genes underlying chicken follicle selection. A total of 2,436 differentially expressed genes (DEGs), 3,852 differentially expressed transcripts (DETs) and 925 differentially expressed lncRNA transcripts were identified between chicken Pre-GCs and Post-GCs. For all of the significant DETs, the alternative 3'splice sites (A3) accounted for a maximum of 23.74% of all alternative spli

SUBMITTER: Li D 

PROVIDER: S-EPMC9877231 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature