Capturing marine microbiomes and environmental DNA: A field sampling guide.

ABSTRACT: The expanding interest in marine microbiome and eDNA sequence data has led to a demand for sample collection and preservation standard practices to enable comparative assessments of results across studies and facilitate meta-analyses. We support this effort by providing guidelines based on a review of published methods and field sampling experiences. The major components considered here are environmental and resource considerations, sample processing strategies, sample storage options, and eDNA extraction protocols. It is impossible to provide universal recommendations considering the wide range of eDNA applications; rather, we provide information to design fit-for-purpose protocols. To manage scope, the focus here is on sampling collection and preservation of prokaryotic and microeukaryot

SUBMITTER: Patin NV 

PROVIDER: S-EPMC9877356 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature