Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19.

ABSTRACT:

Background and purpose

Neutrophil overstimulation plays a crucial role in tissue damage during severe infections. Because pathogen-derived neuraminidase (NEU) stimulates neutrophils, we investigated whether host NEU can be targeted to regulate the neutrophil dysregulation observed in severe infections.

Experimental approach

The effects of NEU inhibitors on lipopolysaccharide (LPS)-stimulated neutrophils from healthy donors or COVID-19 patients were determined by evaluating the shedding of surface sialic acids, cell activation, and reactive oxygen species (ROS) production. Re-analysis of single-cell RNA sequencing of respiratory tract samples from COVID-19 patients also was carried out. The effects of oseltamivir on sepsis and betacoronavirus-induced acute lung injury were ev

SUBMITTER: de Oliveira Formiga R 

PROVIDER: S-EPMC9877938 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature