Microfluidic Platform for Profiling of Extracellular Vesicles from Single Breast Cancer Cells.

ABSTRACT: Extracellular vesicles (EVs) are considered as valuable biomarkers to discriminate healthy from diseased cells such as cancer. Passing cytosolic and plasma membranes before their release, EVs inherit the biochemical properties of the cell. Here, we determine protein profiles of single EVs to understand how much they represent their cell of origin. We use a microfluidic platform which allows to immobilize EVs from completely isolated single cells, reducing heterogeneity of EVs as strongly seen in cell populations. After immunostaining, we employ four-color total internal reflection fluorescence microscopy to enumerate EVs and determine their biochemical fingerprint encoded in membranous or cytosolic proteins. Analyzing single cells derived from pleural effusions of two different human adeno

SUBMITTER: Nikoloff JM 

PROVIDER: S-EPMC9878503 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature